Q5 Hot Start High-Fidelity DNA Polymerase kit by New England Biolabs for DNA amplification to increase sensitivity of genetic analysis during the PCR testing workflow, as used at the Royal London Hospital by the COVID-19 Genomics UK Consortium (COG-UK), a group of public health agencies and academic institutions created in April 2020 to collect, sequence and analyse genomes of SARS-CoV-2 as part of COVID-19 pandemic response, 2020-22.