Florado Muybridge was the son of motion photographer Eadweard Muybridge. Florado Muybridge was born in 1873 to Eadweard Muybridge and Flora Shallcross Stone Muybridge. After doubts over Muybridge's paternity, Eadweard Muybridge shot dead Major Harry Larkyns, suspected to be the true father of Florado. Eadweard Muybridge was acquitted of the Muybridge.

Following divorce proceedings, Flora Shallcross Stone received custody of Florado Muybridge. Stone died in 1875, after which Florado was placed in the care of a French couple. He was later moved to a Catholic orphanage and then a Protestant orphanage (by request of his father).

Photographs of Florado Muybridge as an adult show him to have strongly resembled Muybridge. Put to work on a ranch as a boy, he worked all his life as a ranch hand and gardener. In 1944, Florado was hit by a car in Sacramento and killed, at approximately the age of 70.